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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Signal. 2010 Feb 17;22(7):1013–1021. doi: 10.1016/j.cellsig.2010.02.003

Fig. 6.

Fig. 6

Effect of Reg1-IF point mutations on Snf1 interaction. HA-tagged Snf1 and associated proteins were collected by immunoprecipitation from extracts expressing wild type V5-tagged Reg1 or V5-tagged Reg1-IF. Cells were grown in either high glucose (H) or low glucose (L) prior to protein extraction. Bound Reg1 proteins and the levels of Snf1 and Reg1 proteins in the protein extracts were determined by quantitative western blotting. Extracts were prepared and analyzed in triplicate. (A) Representative blots are shown. (B) Mean integrated band intensity values from triplicate samples are plotted with the error bars representing one standard error. Statistical analysis of the data for associated Reg1 in low glucose using Student’s t test. **, p < 0.001.