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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Signal. 2010 Feb 17;22(7):1013–1021. doi: 10.1016/j.cellsig.2010.02.003

Fig. 7.

Fig. 7

Requirements for Snf1-Reg1 association. (A) Association of Snf1 and Reg1 was measured by coimmunoprecipitation. Reg1 plasmids expressed with wild type (WT) or Reg1-IF proteins. Snf1 plasmids expressed either wild type or Snf1 with the K84R or T210A point mutations. (B) The requirement for Snf1 phosphorylation was examined by coimmunoprecipitation from cells lacking all three Snf1-activating kinases. Extracts were prepared from cells transformed with a plasmid that expresses Sak1 or empty vector. (C) The phosphorylation status of the Snf1 activation loop in the cells used in panels A and B was measured using a phosphopeptide specific antibody (αPT210).