Table 1.
miRNAs that showed different expression among strains GK, WKY and BN in three insulin target tissues
| Tissue | miRNA | Rat chromosome location | Accession number | Location | Strand | p value | Comparison | Validation | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GK/BN | BN/WKY | GK/WKY | ||||||||||||
| Start | End | Fold change | p value | Fold change | p value | Fold change | p value | |||||||
| Adipose | rno-miR-140* | 19 | MI0000611 | 37422704 | 37422802 | + | 1.4 × 10−2 | 1.20 | 2.6 × 10−1 | −1.45 | 3.9 × 10−3 | −1.22 | 4.2 × 10−1 | |
| Adipose | rno-miR-207 | 5 | MI0003479 | 58101186 | 58101263 | + | 2.1 × 10−2 | −1.39 | 9.3 × 10−2 | 1.55 | 8.1 × 10−3 | 1.11 | 8.6 × 10−1 | |
| Adipose | rno-miR-21 | 10 | MI0000850 | 74864500 | 74864591 | − | 4.7 × 10−4 | 1.11 | 5.7 × 10−1 | −1.76 | 3.3 × 10−4 | −1.59 | 8.6 × 10−3 | |
| Adipose | rno-miR-222 | X | MI0000962 | 14935056 | 14935158 | + | 4.7 × 10−4 | 2.43 | 1.4 × 10−4 | −1.70 | 3.9 × 10−3 | 1.42 | 2.1 × 10−1 | † |
| Adipose | rno-miR-27a | 19 | MI0000860 | 25638578 | 25638664 | − | 5.5 × 10−3 | 1.74 | 5.1 × 10−3 | −1.52 | 1.4 × 10−2 | 1.15 | 8.4 × 10−1 | † |
| Adipose | rno-miR-29a | 4 | MI0000863 | 58107760 | 58107847 | − | 7.9 × 10−4 | 1.18 | 2.8 × 10−1 | −1.68 | 3.3 × 10−4 | −1.42 | 4.0 × 10−2 | |
| Adipose | rno-miR-335 | § | MI0000612 | – | – | n/a | 5.5 × 10−3 | 1.96 | 3.0 × 10−2 | −2.24 | 3.6 × 10−3 | −1.14 | 9.0 × 10−1 | |
| Adipose | rno-miR-347 | § | MI0000635 | – | – | n/a | 6.2 × 10−3 | −1.66 | 3.0 × 10−2 | 1.85 | 3.8 × 10−3 | 1.11 | 9.0 × 10−1 | |
| Adipose | rno-miR-487b | 6 | MI0003547 | 1.34 × 108 | 1.34 × 108 | + | 1.2 × 10−3 | −1.40 | 5.1 × 10−3 | 1.43 | 1.1 × 10−3 | 1.02 | 9.5 × 10−1 | |
| Liver | rno-miR-100 | 8 | MI0000885 | 44518670 | 44518749 | + | 3.7 × 10−3 | −1.00 | 9.8 × 10−1 | −1.41 | 2.7 × 10−3 | −1.41 | 4.2 × 10−3 | |
| Liver | rno-miR-103 | 10 | MI0000888 | 20695027 | 20695112 | + | 4.1 × 10−2 | 1.21 | 6.0 × 10−2 | −1.16 | 9.2 × 10−2 | 1.05 | 9.6 × 10−1 | † |
| Liver | rno-miR-125b | 8 | MI0000896 | 44570155 | 44570241 | + | 3.7 × 10−3 | −1.09 | 5.3 × 10−1 | −1.33 | 5.7 × 10−3 | −1.44 | 3.7 × 10−3 | |
| Liver | rno-miR-126 | 3 | MI0000898 | 4768117 | 4768189 | + | 3.3 × 10−2 | −1.40 | 6.0 × 10−2 | 1.43 | 2.4 × 10−2 | 1.02 | 9.9 × 10−1 | |
| Liver | rno-miR-140* | 19 | MI0000611 | 37422704 | 37422802 | + | 3.5 × 10−2 | 1.14 | 3.9 × 10−1 | −1.32 | 1.5 × 10−2 | −1.16 | 3.6 × 10−1 | |
| Liver | rno-miR-146 | 10 | MI0000919 | 28476368 | 28476462 | − | 7.0 × 10−3 | 1.24 | 6.0 × 10−2 | −1.36 | 4.2 × 10−3 | −1.10 | 6.4 × 10−1 | |
| Liver | rno-miR-15b | 2 | MI0000843 | 1.59 × 108 | 1.59 × 108 | + | 7.0 × 10−3 | −1.20 | 6.0 × 10−2 | 1.32 | 4.2 × 10−3 | 1.10 | 6.1 × 10−1 | |
| Liver | rno-miR-191 | 8 | MI0000934 | 1.14 × 108 | 1.14 × 108 | + | 3.3 × 10−2 | 1.27 | 6.0 × 10−2 | −1.02 | 8.7 × 10−1 | 1.25 | 6.9 × 10−2 | |
| Liver | rno-miR-193 | 10 | MI0000936 | 65901048 | 65901133 | + | 3.5 × 10−2 | 1.32 | 6.0 × 10−2 | 1.00 | 1.0 | 1.32 | 6.9 × 10−2 | |
| Liver | rno−miR-195 | 10 | MI0000939 | 57074170 | 57074256 | + | 6.2 × 10−3 | 1.65 | 6.1 × 10−3 | −1.21 | 2.0 × 10−1 | 1.37 | 6.9 × 10−2 | † |
| Liver | rno-miR-200a | 5 | MI0000943 | 1.73 × 108 | 1.73 × 108 | − | 7.0 × 10−3 | −1.55 | 6.0 × 10−2 | −1.17 | 4.4 × 10−1 | −1.81 | 6.0 × 10−3 | |
| Liver | rno-miR-222 | X | MI0000962 | 14935056 | 14935158 | + | 2.3 × 10−2 | 1.17 | 1.9 × 10−1 | −1.35 | 8.0 × 10−3 | −1.15 | 3.6 × 10−1 | |
| Liver | rno-miR-27b | 17 | MI0000859 | 7351449 | 7351545 | − | 3.5 × 10−2 | −1.24 | 1.1 × 10−1 | 1.31 | 2.3 × 10−2 | 1.06 | 9.6 × 10−1 | |
| Liver | rno-miR-290 | 1 | MI0000964 | 64274926 | 64275006 | − | 3.7 × 10−3 | 1.13 | 5.7 × 10−1 | −1.87 | 2.7 × 10−3 | −1.66 | 1.4 × 10−2 | |
| Liver | rno-miR-292-5p | 1 | MI0000966 | 64274340 | 64274421 | − | 3.2 × 10−2 | 1.05 | 9.1 × 10−1 | −2.17 | 1.8 × 10−2 | −2.07 | 6.9 × 10−2 | |
| Liver | rno-miR-296 | 3 | MI0000967 | 1.65 × 108 | 1.65 × 108 | − | 2.3 × 10−2 | 1.11 | 5.3 × 10−1 | −1.39 | 8.0 × 10−3 | −1.25 | 1.6 × 10−1 | |
| Liver | rno-miR-335 | § | MI0000612 | – | – | n/a | 1.1 × 10−2 | −1.05 | 7.0 × 10−1 | 1.38 | 5.7 × 10−3 | 1.31 | 4.7 × 10−2 | |
| Liver | rno-miR-422b | 18 | MI0003719 | 57377805 | 57377869 | − | 3.2 × 10−2 | −1.22 | 1.4 × 10−1 | 1.36 | 1.3 × 10−2 | 1.11 | 7.0 × 10−1 | |
| Skeletal muscle | rno−let-7e | 1 | MI0000832 | 56487134 | 56487226 | + | 3.5 × 10−3 | 1.04 | 8.5 × 10−1 | −1.36 | 4.0 × 10−3 | −1.30 | 2.0 × 10−2 | |
| Skeletal muscle | rno-miR-10b | 3 | MI0000842 | 57340853 | 57340961 | + | 3.5 × 10−3 | −1.59 | 1.5 × 10−3 | 1.14 | 4.0 × 10−1 | −1.40 | 2.6 × 10−2 | † |
Rno-miR-422b is now called rno-mir-378 (accession number MI0003719; located on rat chromosome 18).
Strain differences in expression were detected by ANOVA, All p value s are adjusted for FDR = 5%
§Indicates that genomic microRNA location is unknown (rat chromosome location column)
†miRNAs for which quantitative RT-PCR was attempted
n/a, strand unknown