Fig. 5.

Effect of cold stress on expression of Ucp1 mRNA and methylation of CpG dinucleotides in the Ucp1 enhancer in different tissues from mice. a Ucp1 mRNA abundance normalised to 18S rRNA in iBAT, iWAT, gWAT and liver from mice housed in either warm (22 ± 2°C, white bars) or cold (8 ± 2°C, black bars) conditions for 24 h before sampling. b Methylation of CpG dinucleotides in positions 1 to 6 of the Ucp1 enhancer in tissues from mice housed in a warm environment. c Methylation of CpG dinucleotides in positions 1 to 6 of the Ucp1 enhancer in tissues from mice housed in a cold environment: iBAT (black bars), iWAT (white bars), gWAT (bars with horizontal stripes) and liver (bars with vertical stripes). Bars represent mean ± SEM (n = 4 mice). Significant difference between warm and cold, **p < 0.01