Fig. 1.

Effect of spermine nitric oxide donor on glucose transport, cGMP content and AMPK activity in human skeletal muscle. Skeletal muscle strips from seven healthy men were incubated in the absence (basal) or presence of spermine NONOate (Spe-NO, nitric oxide donor; 5 mmol/l) or insulin (120 nmol/l). Glucose transport (a), intracellular cyclic GMP (cGMP) content (b), and both AMPK-α1 (c) and AMPK-α2 (d) isoform-specific activity were determined. e AMPK-α1 and AMPK-α2 (f) isoform-specific activity in skeletal muscle from healthy men (n = 2) in response to acute exercise. Results are expressed as mean ± SEM. *p < 0.05, **p < 0.01 vs basal