Figure 4.

The effects of L1 cytoplasmic domain mutations on L1-mediated neurite outgrowth. L1KO neurons were transfected with missense mutations and grown on an L1 substrate. Total neurite length, longest neurite length, and branching number were quantified. The mean ± SEM values of the mutant-transfected neurons were always normalized by the mean values of neurons transfected with WT hL1 in the same experiment. Values shown are the average of the mean ± SEM percentage values from three experiments. ANOVA (Fisher's PLSD) was done using Statview 4.5. Statistical significance is shown. ^***p < 0.001 in all three experiments; ^**p < 0.05 in all three experiments; ^*p < 0.05 in two of the three experiments.