Figure 7.

Both the juxtamembrane region and the RSLE region are critical for the L1-ERM interaction. The pAS2 bait vectors containing wild-type and mutant forms of the L1CD fused to the Gal4 DNA-binding domain are cotransformed with the prey vector pACT2 containing ezrin (A), Drosophila ankyrin (B), or μ2 subunit of AP-2 (C) fused to the Gal4 activation domain and tested for interactions on histidine-deficient plates containing 10 mm 3-AT (A, B) or 5 mm 3-AT (C). Each plate includes one positive control p53+SV40, which is a diploid of AH109 [pGBKT7-53] (p53 was fused to GAL4 DNA-binding domain), and Y187 [pTD1-1] (SV40 large T antigen was fused to GAL4 activation domain), because the p53 and the SV40 large T antigen was known to interact in the yeast two-hybrid assay. A negative control was included on each plate, which is a diploid of AH109 [pAS2-L1CD] and Y187 [pTD1-1].