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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: J Immunol. 2009 Oct 28;183(10):6460–6468. doi: 10.4049/jimmunol.0900983

FIGURE 7.

FIGURE 7

A, Schematic depicting the loss of FRET between αM-mCFP and β2-mYFP during Mac-1 cytoplasmic tail separation. B, K562 cells expressing αM-mCFP and β2-mYFP were treated with 1 mM MnCl2 in the absence or presence of 10 µg/ml CBR LFA-1/2 Fab or 200 µg/ml ICAM-1, or with 100 nM PMA. CFP and YFP images were captured of individual cells before and after photobleaching of the YFP signal. FRET efficiency was calculated as described in Materials and Methods. *, Significantly different from control (p < 0.05).