Table 1.
Oligonucleotide primer and probe sequences used for real-time PCR.
| Gene | Sequence |
|---|---|
| 18S | Forward: 5′-GCCGCTAGAGGTGAAATTCTTG-3′ Reverse: 5′-CATTCTTGGCAAATGCTTTCG-3′ Probe: 5′-ACCGGCGCAAGACGGACCAG-3′ |
| IL-1β | Forward: 5′-CAGGCTCCAGGATGCACAA-3′ Reverse: 5′-AGGTGGAGAGCTTTCAGCTCAT-3′ Probe: 5′-AGAAATCCCTGGTGTTGTCTGGCACG-3′ |
| Keratin 5 | Forward: 5′-TGCCTCCTTCATTGACAAGGT-3′ Reverse: 5′-CAGCAGCTCCCATTTCGTTT-3′ Probe: 5′-CGGTTCCTGGAGCAGCAGAACCA-3′ |
| Keratin 10 | Forward: 5′-CAGGATGAAGTATGAGAACGAGGTT-3′ Reverse: 5′-TGCGCAGCCCGTTGAT-3′ Probe: 5′-CCCTGCGCCAGAGCGTGGAG-3′ |
| TGF-β1 | Forward: 5′-TGCGGCAGCTGTACATTGAC-3′ Reverse: 5′-GGCAGAAGTTGGCGTGGTA-3′ Probe: 5′-AAGGACCTGGGCTGGAAGTGGATCC-3′ |
| TGF-β3 | Forward: 5′-CGGCTCAAGAAGCAGAAGGA-3′ Reverse: 5′-CGGTGCGGTGGAATCATC-3′ Probe: 5′-CACCACAACCCTCACCTCATCCTC-3′ |
| TNF-α | Forward: 5′-TGGTCACCCTCAGATCAGCTT-3′ Reverse: 5′-CTTGCGGGTTTGCTACTACGT-3′ Probe: 5′-TCGGGCCCTGAGTGACGAGCC-3′ |
| SMAD 3 | Forward: 5′-CATCGAGCCCCAGAGCAATA-3′ Reverse: 5′-GTCTCTCCATCTTCGCTCAGGTA-3′ Probe: 5′-TGGCTTCTGTCCTGTGGACATTGGAG-3′ |
| SMAD 7 | Forward: 5′-GATTTTCTCAAACCAACTGCAGATT-3′ Reverse: 5′-TAATTCGTTCCCCCTGTTTCA-3′ Probe: 5′-TCCAGATGCTGTGCCTTCCTCCG-3′ |
| Vimentin | Forward: 5′-ACAACCTGGCCGAAGACATC-3′ Reverse: 5′-GCTTCCTCTCTCTGCAGCATCT-3′ Probe: 5′-TGCGGCTCCGGGAGAAGTTGC-3′ |