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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Eurographics Workshop Vis Comput Biomed. 2008 Jan 1;2008:151–158. doi: 10.2312/VCBM/VCBM08/151-158

Figure 3.

Figure 3

Examples of unintuitive images in fluorescence microscopy. (a) Models of surface-labeled small tubes. The side-by-side tubes are half the diameter of the larger tube. (b) Simulated noise-free fluorescence image of the tubes convolved with a calculated widefield PSF generated by XCOSM [MC02]. The two tubes can easily be misconstrued as a single tube while the single tube appears as two tubes. (c) A surface-labeled bead (in wireframe) with 1 micron radius superimposed with experimental images at focal planes with 2 micron spacing. The top-most image could be interpretted as the top of the sphere, but it is four times further away from the sphere center than the true top.