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. 2010 Apr 20;7(2):72–81. doi: 10.7150/ijms.7.72

Figure 4.

Figure 4

Unlabeled target cell- or anti-MHC class I monoclonal antibody-mediated blocking of cytotoxicity by MLPC cells. A CFSE-labeled target cell cytotoxicity assay was performed by using MLPC cells as effector cells and autologous B-LCL pulsed with modified-type WT1 peptides as target cells. CFSE-labeled B-LCL were mixed with 5-20 times unlabeled B-LCL (cold target) and co-cultured with MLPC cells at effector : the CFSE-labeled target ratio of 3:1 for the cold target inhibition test. The same CFSE-labeled target cells were incubated with anti-MHC class I or II monoclonal antibodies at 10 μg/ml for 30 min and then co-cultured with MLPC cells at effector : target ratio of 3:1 for the anti-MHC class I monoclonal antibody inhibition test. This is a representative experiment among four similar experiments. The other three similar experiments induced the identical results as one inserted in Figure 5.