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Cytotoxicity assay of MLPC cells against leukemia cells expressing WT1 intrinsically. A CFSE-labeled target cytotoxicity assay was performed by using cells cultured in each well of MLPC for 2 weeks as effector cells and a CML blastic crisis cell line expressing HLA-A*2402, C2F8, as target cells at effector : target ratio of 5:1. MLPC was initiated by using MNCs of PB drawn from a CML patient at 3 weeks after the 2nd administration of WT1 peptide vaccination.
