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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Neurochem. 2010 Mar 4;113(4):1023–1035. doi: 10.1111/j.1471-4159.2010.06666.x

Figure 7.

Figure 7

Effect of PFC ibotenic acid or basal forebrain 192 IgG-saporin lesions on vGlut1 or vAChT immunoreactivity in the LH/PFA. A. Ibotenic acid lesions of the PFC reduced the number of apparent appositions between vGlut1-immunoreactive varicosities and orexin neurons. B & C. Double-label immunohistochemistry for vGlut1 (dark puncta) and orexin A (light brown cell somata) from a sham (B) or ibotenic acid-treated rat (C). Arrows indicate points of apparent appositional contact. D. The cholinergic immunotoxin 192 IgG-saporin in the basal forebrain reduced the number of apparent appositions between vAChT-immunoreactive varicosities and orexin neurons. E & F. Double-label immunohistochemistry for vAChT (dark puncta) and orexin neurons (light brown somata) from a sham (E) or 192 IgG-saporin-treated (F) rat. Arrows indicate apparent appositional contacts. Scale bars, approximately 20 μm. *P < 0.05 vs. sham-lesioned group.