Accumulation and extrusion of Flutax-2 in tissues. (A) Flutax-2 accumulation in cell suspension of tissues. Cell suspensions of NCI/ADR-RES tumor, small intestine and liver of athymic mice were incubated with Flutax-2 (1 μM) in medium containing collagenase IV. The smear slides from each incubation condition were observed under microscope (left side, bright field; right side, fluorescence filed). (B) Accumulation and extrusion of Flutax-2 in cell suspensions of tumor, small intestine and liver. Increasing amounts of cell suspensions were incubated with Flutax-2 (1 μM) in medium containing collagenase. After 2 hr, cellular Flutax-2 was measured to evaluate accumulation (dashed line). The extrusion of the suspensions was measured after 2 hr additional incubation (solid line).