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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2010 Feb 12;1802(6):545–551. doi: 10.1016/j.bbadis.2010.02.003

Figure 2.

Figure 2

Processivity of mutant and wild type human POLGα subunit. DNA synthesis by mutant POLGα was measured at 30 mM KCl on singly primed M13 DNA as described in Materials and Methods. DNA product strands were isolated, denatured and electrophoresed as duplicates in a 6% denaturing polyacrylamide gel. Lanes 1 and 2, wild type POLGα; lanes 3 and 4, W748S mutant POLGα. Size marker: NEB 2-log DNA-ladder (molecular weights shown). The processivity of the mutant catalytic core enzyme was similar to the wild type enzyme.