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. 2009 Dec 14;425(Pt 1):159–168. doi: 10.1042/BJ20090884

Figure 3. Translocation of STIM1–EYFP is inhibited by the depletion of multiple phosphoinositides in cells treated with LY294002.

Figure 3

HeLa cells were co-transfected with the STIM1-EYFP, PM-FRB-RFP and RFP-ptase-dom constructs. (A) Cells were perfused with no additions (controls), or 50 or 300 μM LY294002 for 30 min followed by addition of thapsigargin, which resulted in some STIM1 translocation into puncta under all conditions. Note that after treatment with 300 μM LY294002, few ST1M1 puncta formed in some cells (e.g. the cell marked by an asterisk). (B) Cells were perfused with 50 μM LY294002 for 30 min followed by addition of rapamycin. Treatment with thapsigargin had little effect on the distribution of STIM1. (C) Cells were perfused with 300 μM LY294002 for 30 min followed by addition of rapamycin. Treatment with thapsigargin had little effect on the distribution of STIM1. Scale bar, 10 μm. Ly29, LY294002; Rapa, rapamycin; Thapsi, thapsigargin.