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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Nat Neurosci. 2010 Apr 11;13(5):567–576. doi: 10.1038/nn.2528

Figure 4. Altered composition of autophagic–related compartments in HD cells.

Figure 4

(a) Electron micrographs of fractions enriched in autophagosomes and autophagolysosomes isolated from liver of 18Qhtt and 111Qhtt mice. Insets: higher magnification images of single vesicles. Right: Percentage of vesicles with an electron–clear (light), vesicular (multivesicular) or electron–dense content (dark). Mean+s.e.m of 3 different isolations (estimated > 1,000 AVs). (b) Bidimensional electrophoresis and SyproRuby staining of the same fractions. Samples on the right are the content of the same fractions isolated in the supernatant after hypotonic shock and high speed centrifugation. * p < 0.05.