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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: J Vasc Surg. 2010 Mar 11;51(5):1248–1259. doi: 10.1016/j.jvs.2009.12.028

Figure 2.

Figure 2

IPA/NO prevents HUVEC proliferation in vitro, but does not cause cell death or affect migration. (A) Proliferation of HUVEC was assessed using 3H-thymidine incorporation. (B) HUVEC death was assessed via Guava PCA after 24 hours of treatment with IPA/NO. (C) The effect of IPA/NO (1000 μmol/L) on HUVEC migration was assessed by blinded counting of nuclei migrating into the scraped area. The effects of the parent compound, IPA, and breakdown product, isopropanol, of IPA/NO on proliferation, death and migration were also assessed at 1000 μmol/L. *P<0.005 vs. control. n = 3/treatment group. Data shown are representative of three separate experiments.