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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Cell Signal. 2010 Mar 3;22(7):1076–1087. doi: 10.1016/j.cellsig.2010.02.010

Fig. 6.

Fig. 6

Regulation of apoptosis in HSF. (A) Pretreatment with PPP or anti-IL-33 mAb increased radiation-induced or TRAIL-induced apoptosis levels in directly exposed or bystander cells. Apoptosis was induced by TRAIL (50 ng/ml) and cycloheximide (CHX, 2 μg/ml) in control, directly irradiated and bystander cells pretreated with nonspecific IgG, anti-IL-33 (2 μg/ml) mAb or PPP (0.5 μM). Apoptotic levels were determined using PI staining of DNA and FACS analysis. (B) Western blot analysis of indicated proteins from control, directly irradiated and bystander cells. FOXO3A levels were used as protein loading controls. (C) Surface expression of TRAIL-R2/DR5 was determined using immuno-staining and FACS analysis of control, α-irradiated and bystander HSF 16 h after treatment.