Validation of the kinetics and binding affinity of the 3.L2-D73/I-Ek interaction. A. Sample SPR sensorgram used to determine kinetic binding parameters for the 3.L2-D73/I-Ek interaction ([3.L2] are 200, 109.5, 54.8, 27.4, 13.7, 6.9, and 3.5 μM from top to bottom). Sensorgram shown is representative of four experiments. B. Equilibrium binding analysis of the 3.L2-D73/I-Ek interaction to determine the equilibrium KD. Data are presented as the mean ± standard deviation, and plots are representative of four experiments.