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Displacement of FeIII from holoFbp (25 μM) by 1 : 50 Nb : NTA under the same conditions as Figure 2(a). Molar ratio of Nb/Fbp (from top to bottom) is 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, and 33. insert shows the percentage of FeIII bound to the protein calculated from the LMCT band at 465 nm. Little FeIII is displaced by [Nb(NTA)2]− (open circles) whereas 1 : 50 Nb : NTA gives rise to almost complete displacement of FeIII from holoFbp (filled circles).
