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Figure 2. Isolation of the two isomers of compound 12 by JAI LC-918 recycling preparative HPLC.
Mobile phase: 85% Acetonitrile in water (0.1% TFA); Flowrate: 4.0 mL/min; Detector: Refractive Index (RI); Column: Alltima 10mm × 250mm C18 5μ. Peak 1 is 12a (3′S isomer); and peak 2 is 12b (3′R isomer).