Figure 2.

Time course of the effects of N or P limitation on the expression of 1, 2, and 3 in N. punctiforme. At time zero vegetative cells of N. punctiforme were inoculated into fresh medium. Following 7 days of growth, at the time indicated by the arrow, cells were harvested, washed and resuspened in either complete medium (△) or medium lacking either P (□) or N (○). Data points are the means of three replicates and error bars represent the standard deviation of the means.