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Time course of N or P limitation on the ratio of 2-methylhopanoid to desmethylhopanoids (i.e. 2-methylhopanoid index). At time zero, vegetative cells of N. punctiforme were inoculated into fresh medium. Following one week (arrow), cells were harvested, washed and resuspened in either complete medium (△) or medium lacking either P (□) or N (○).
