Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2010 Jan 20;48(7):905–914. doi: 10.1016/j.freeradbiomed.2010.01.015

Figure 1. Measurement of Mitochondrial Function in BAEC using the XF24 analyzer.

Figure 1

Seahorse Bioscience V7 Tissue Culture Plates were seeded with BAEC (20,000–60,000 cells/well) and allowed to grow for 24 h before the measurement of OCR and ECAR. Panel A: Basal OCR and ECAR were measured three times and plotted as a function of cell seeding number. Panel B represents the time course for measurement of OCR for 40,000 cells under the basal condition followed by the sequential addition of oligomycin (1 µg/ml), FCCP (1 µM), and antimycin A (10 µM) with a measurement of OCR and ECAR as indicated. This progress curve is annotated to show the relative contribution of non-respiratory chain oxygen consumption, ATP-linked oxygen consumption, the maximal OCR after the addition of FCCP, and the reserve capacity of the cells. The contribution of each of these parameters to the total cellular oxygen consumption is plotted in Panel C. All data are the mean ± sem, n≥3 per group. *, p<0.05 vs. Control; #, p<0.05 vs. Maximal OCR.