Figure 3. Bioenergetic profile of BAEC treated with inhibitors of mitochondrial oxygen consumption.
BAEC were seeded at 40,000 cells/well and treated with 10 µM Antimycin A, 1 µg/ml oligomycin, 1 µM rotenone, 10 µM TTFA, 1 mM cyanide, or 10 µM myxothiazole and OCR and ECAR were measured. The resulting effects on OCR and ECAR are plotted as a percentage of the baseline measurement for each treatment. Data shown are the mean ± sem. n≥3 per treatment group.