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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2010 Jan 20;48(7):905–914. doi: 10.1016/j.freeradbiomed.2010.01.015

Figure 8. Reversal of OCR Inhibition is achieved following removal of Deta NONOate or DMNQ, but not these treatments combined.

Figure 8

BAEC were seeded at 40,000 cells/well and allowed to grow for 24 h. Three baseline measurements of OCR were made, and then cells were treated with Deta NONOate (250 µM), DMNQ (15 µM), or both for 1 h. Following this treatment, three further measurements of OCR were performed. The plate was removed from the XF24, and the media changed to fresh assay media in the indicated groups. Three further measurements of basal OCR were then performed prior to sequential injection of oligomycin (1µg/ml), FCCP (1µM), and antimycin A (10 µM) to determine mitochondrial function. This portion of the experiment is shown in Panel A. Panel B: Reserve capacity was calculated for cells in the presence of Deta NONOate, or with the NO donor removed. Panel C: Reserve capacity was calculated for cells in the presence of DMNQ, or with the DMNQ removed. Panel D: Reserve capacity was calculated for cells in the presence of Deta NONOate and DMNQ combined, or with these compounds removed. Data shown are the mean ± sem, n=5 per group. *, p<0.05 vs. Control. §, p<0.05 vs. Wash control.