Table 2.

Genotyping assays for selected variants in eight folate-related genes

Gene Name	Gene Abbreviation	Polymorphism	dbSNP-rs#	AA*- position	Assay	Forward/Reverse Primer/Vic-reporter or AOD Reference Number*
5,10-methylene tetrahydrofolate reductase	MTHFR	A1298C	1801131	E429A	ABD	GGAGGAGCTGCTGAAGATGTG CCCGAGAGGTAAAGAACAAAGACTT ACCAGTGAAGAAAGTGT
5,10-methylene tetrahydrofolate reductase	MTHFR	C677T	1801133	A222V	AOD‡	C____1202883_20*
Methionine synthase	MS/MTR	A2756G	1805087	D919G	AOD	C____12005959_10*
Methionine synthase reductase	MTRR	A66G	1801394	I22M	AOD	C____3068176_10*
Monocyte chemotactic protein-1	MCP1	(-A2518G)	1024611	UTR	ABD	TTCTTGACAGAGCAGAAGTGG GCCTTTGCATATATCAGACAGTA AGACAGCTGTCACTTTC
Betaine-homocysteine methyltransferase	BHMT	G742A	3733890	R239Q	ABD†	CTCATGAAGGAGGGCTTGGA CCAAGGGCTGGCTCATCAG CTTTCAGTTGGGCAGC
Transcobalamin II	TCN2	C777G	1801198	P259R	AOD	C____325467_10*
Serine Hydroxymethyltransferase	SHMT	C1420T	1979277	F474L	ABD	CTCCGGGAGGAGGTTGAGA GCCCGCTCCTTTAGAAGTCA CTTCGCCTCTCTCTTC
Endothelial nitric oxide syntthase	NOS3	G894T	1799983	E298D	EPOCH	ACGGCTGGACCCCAGGAAA ACCAGCTCGGG*GGGCAGAA CCCCAGATGATCC

^*AA, amino acid;

^†ABD, assay by design;

^‡AOD, assay on demand;

^§EPOCH