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Transient expression of GFP fusion proteins in Arabidopsis thaliana protoplasts. The Dim1A and Dim1B gene fragments encoding putative transit peptides were inserted into plasmid pOL–GFPS65C to generate vectors driving the expression of Dim1A–GFP (a) and Dim1B–GFP (b) showing nuclear (a) and mitochondrial (b) GFP localisation. Control constructs code for plastidial RecA–GFP (c) and mitochondrial CoxIV–GFP (d), respectively. Images were taken by confocal fluorescence microscopy.
