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. Author manuscript; available in PMC: 2010 Apr 28.
Published in final edited form as: Mol Psychiatry. 2009 Feb 10;14(11):1004–1016. doi: 10.1038/mp.2009.10

Figure 3.

Figure 3

Overexpression of IL-33 in the neuroblastoma SKNSH-SY5Y cell line stably transfected with APP695wt. (A) Representative experiment of APP metabolism variations following transfection of the IL-33 expression vector (APPs, APP soluble; APP-CTF, APP carboxy terminal fragments); (B) Representative experiment of Aβ40 and Aβ42 secretions following transfection of the IL-33 expression vector; (C) Mean variations of the Aβ40 and Aβ42 secretions from three independent experimentations in duplicates; (D) IL-33 accumulates in nucleus (colocalisation with dense regions of DAPI staining, indicating association with heterochromatin). No signal was detected using an empty vector (Mock) (Data not shown); (E) detection of the ST2 receptor expression in the SY5Y-APP695wt cell line by immunofluorescence experiments and western blot; (F) Representative experiment of APP metabolism variations in response to human recombinant IL-33 protein; (G) Representative experiment of Aβ40 and Aβ42 secretions in response to human recombinant IL-33 protein; (H) Mean variations of the Aβ40 and Aβ42 secretions from three independent experimentations in duplicates.