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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: FEBS J. 2010 Jan 18;277(4):951–963. doi: 10.1111/j.1742-4658.2009.07538.x

Figure 1.

Figure 1

A. Dual-luciferase reporter gene expression of APOH promoter deletion mutants in COS – 1 cells. Left panel, schematic representation of 5'-deleted fragments of the APOH promoter in conjunction with the luciferase gene in pGL3-basic vector. The nucleotides are numbered from the translation start site (ATG). The effect of wild-type and mutants were measured as the mean of the firefly luciferase levels, which were normalized by the Renilla luciferase activity, which served as the reference for the transfection efficiency. The results presented are from one out of three independent experiments. pGL3-B indicates the promoterless vector. Asterisk (*) indicates that Del mutant 5 has significantly lower luciferase activity than the wild type (P<0.001).

B. Dual-luciferase reporter gene expression of APOH promoter deletion mutants in HepG2 cells. Left panel, schematic representation of 5'-deleted fragments of the APOH promoter in conjunction with the luciferase gene in pGL3-basic vector. The nucleotides are numbered from the translation start site. The effect of wild-type and mutants were measured as the mean of the firefly luciferase levels, which were normalized by the Renilla luciferase activity, which served as the reference for the transfection efficiency. The results presented are from one out of two independent experiments. pGL3-B indicates the promoterless vector. Asterisk (*) indicates that Del mutant 5 has significantly lower luciferase activity than the wild type (P<0.001).