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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 May;66(1):32–39. doi: 10.1073/pnas.66.1.32

Axonal Growth from Insect Neurons in Glia-Free Cultures*

J S Chen 1,2, R Levi-Montalcini 1,2
PMCID: PMC286083  PMID: 16591833

Abstract

Mechanical dissociation of nerve and glial cells from 16-day embryos of the cockroach Periplaneta americana and their subsequent culture in a Co2-conditioned, chemically defined medium results in the survival of neurons but not of glial cells. The dissociated nerve cells remain alive and in excellent condition for many months and build a dense fibrillar network in presence of foregut explants from the same embryos. These explants are invaded by numerous and large fiber bundles emerging from the fibrillar network. The morphological characteristics of nerve cells and the structural and ultrastructural features of nerve bundles in long-term glia-free culture are described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen J. S., Levi-Montalcini R. Axonal outgrowth and cell migration in vitro from nervous system of cockroach embryos. Science. 1969 Oct 31;166(3905):631–632. doi: 10.1126/science.166.3905.631. [DOI] [PubMed] [Google Scholar]

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