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. Author manuscript; available in PMC: 2010 Dec 11.
Published in final edited form as: J Mol Biol. 2009 Oct 21;394(4):694–707. doi: 10.1016/j.jmb.2009.10.036

Figure 2. Strand Transfer Reactions with RT Titration.

Figure 2

(a) The substrates used are the same as in Figure 1, except that reactions were run either with or without the PCIS. Also, acceptor RNA (gray) was added at the start of the reaction. (b) Reactions with −PCIS or +PCIS substrates were incubated with or without NC for 60 min, and samples were taken. E478Q RT and dNTP were added to the reaction, and incubated for 5 min. RT concentrations were 37, 2.56, 1.28 and 0.64 nM. Donor extension (DE) and strand transfer (TP) products are indicated. (c–f) Average transfer efficiencies determined from strand transfer reactions are represented in the graphs.