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. Author manuscript; available in PMC: 2010 Dec 11.
Published in final edited form as: J Mol Biol. 2009 Oct 21;394(4):694–707. doi: 10.1016/j.jmb.2009.10.036

Figure 3. Donor Cleavage Profiles for RT mutants and WT.

Figure 3

(a) The substrates used are the same as in Figure 1, except that the RNA donor is 5′-radiolabeled, not the DNA primer. (b) Intact donor RNA (D) and primary (1) or secondary (2) cleavage products are generated over time (1, 5, 15 30 min) in the absence (−NC) and presence (+NC) of NC. RNA cleavage products were visualized in denaturing polyacrylamide gel electrophoresis. Gel lanes are numbered 1–20. Lanes 1 - 5 WT RT, 5 - 8 E478Q RT, 9 - 12 H539F RT, 13 - 16 H539D RT, 17 - 20 H539R RT.