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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Gerontol. 2010 Mar 1;45(5):366–374. doi: 10.1016/j.exger.2010.02.009

Figure 1.

Figure 1

Insulin-like growth factor binding protein 5 (Igfbp5) expression in 21 tissues of PappA(−/−) mice and wild type littermates. The chart shows 95% confidence intervals associated with the estimated ratio of expression in PappA(−/−) mice relative to wild type littermates (n = 6 mice per tissue; RT-PCR). Estimates larger than one indicate increased expression of Igfbp5 in PappA(−/−) mice relative to controls, while estimates less than one indicate decreased Igfbp5 expression in PappA(−/−) mice relative to controls. Significant effects are indicated by confidence intervals that do not overlap with the dotted vertical line, which corresponds to an estimated ratio of one (i.e., equal expression in PappA(−/−) and control mice). Calculations were performed using the ΔΔCt method and expression of either 18S ribosomal RNA (Rn18s) or glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as an internal reference gene.