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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Exp Gerontol. 2010 Mar 1;45(5):366–374. doi: 10.1016/j.exger.2010.02.009

Figure 3.

Figure 3

Transcriptional regulation of insulin receptor substrate 1 (Irs1), metallothionein 1 (Mt1) and insulin-like growth factor binding protein acid labile subunit (Igfals) by growth hormone (GH) and/or calorie restriction (CR) in kidney. GH-deficient (Ames) Prop1(df/df) and wild-type mice were injected with either GH or saline for a six-week period. The experiment was performed with mice maintained on either a ad lib or 30% CR diet. The chart shows the relative expression of each gene in each of the eight experimental treatments (n = 10 mice per treatment). The asterisk (*) symbol is used to denote treatments that differ significantly from the baseline treatment (i.e., ad lib fed wild-type mice; P < 0.05, Dunnett post-hoc comparison). Calculations were performed using the ΔΔCt method with expression of 18S ribosomal RNA (Rn18s) used as an internal reference gene.