Table 1.
Hepatic gene expression patterns in PappA(−/−), Ghr(−/−) and Pit1(dw/dw) mice relative to wild-type controls. RT-PCR was used to evaluate relative expression of genes associated with loss of GH / IGF-1 signals, which had been identified in a prior meta-analysis of microarray experiments (Swindell, 2007). The table lists estimated ratios of expression in mutant mice relative to wild-type littermates (n = 6 per mice treatment), with values larger than one indicating increased expression in mutants. Calculations were performed using the ΔΔCt method with expression of 18S ribosomal RNA (Rn18s) as an internal reference gene. P-values in parentheses are based upon results from two-sample t-tests applied to the estimated ΔCt values. Significant results are shown in bold-faced type. The full gene name associated with each gene symbol is provided in Supplemental Data File 1.
| Gene |
PappA(−/−) / WT (P-value) |
Ghr(−/−) / WT (P-Value) |
Pit1(dw/dw) / WT (P-Value) |
|---|---|---|---|
| Igf1 | 1.16 (0.319) | 0.01 (< 0.001) | 0.02 (< 0.001) |
| Igfals | 1.35 (0.073) | 0.03 (< 0.001) | 0.04 (< 0.001) |
| Socs2 | 0.96 (0.927) | 0.25 (0.002) | 0.12 (< 0.001) |
| Lifr | 1.59 (0.070) | 0.74 (0.147) | 0.32 (< 0.001) |
| Egfr | 0.87 (0.358) | 0.11 (< 0.001) | 0.08 (< 0.001) |
| Keg1 | 0.76 (0.062) | 0.03 (< 0.001) | 0.05 (< 0.001) |
| Mup3 | 1.08 (0.627) | 0.09 (< 0.001) | 0.00 (< 0.001) |
| Sult2a2 | 0.92 (0.686) | 2070 (< 0.001) | 696 (< 0.001) |
| Spink3 | 1.92 (0.224) | 104 (< 0.001) | 187 (< 0.001) |
| Fmo3 | 1.34 (0.401) | 28.0 (< 0.001) | 921 (< 0.001) |
| Mt1 | 1.42 (0.451) | 4.91 (0.071) | 30.1 (< 0.001) |
| Mt2 | 1.12 (0.818) | 7.46 (0.080) | 40.3 (< 0.001) |
| Hao3 | 0.59 (0.069) | 8.84 (0.003) | 18.9 (< 0.001) |
| Cyp2a4 | 0.937 (0.795) | 5.27 (< 0.001) | 2.74 (< 0.001) |