Table 2.
Comparison between significant effects of the PappA(−/−) mutation in six organs and gene expression patterns in Ghr(−/−) and Pit1(dw/dw) mice. Our analysis of IGF-associated genes in six organs from the PappA(−/−) mouse identified 16 significant effects of the PappA(−/−) mutation. For each gene and tissue in which a significant effect was found, we evaluated effects of the Ghr(−/−) and Pit1(dw/dw) mutations. The table lists estimated ratios of expression in mutant mice relative to wild-type littermates (n = 6 per mice treatment), with values larger than one indicating increased expression in mutants. Calculations were performed using the ΔΔCt method with expression of either 18S ribosomal RNA (Rn18s) or glyceraldehyde-3-phosphate dehydrogenase (Gapdh) used as an internal reference gene. Unless otherwise indicated, p-values in parentheses are based upon results from two-sample t-tests applied to the estimated ΔCt values. Significant results are shown in bold-faced type. The full gene name associated with each gene symbol is provided in Supplemental Data File 1.
| Tissue | Gene |
PappA(−/−) / WT (P-value) |
Ghr(−/−) / WT (P-Value) |
Pit1(dw/dw) / WT (P-Value) |
|---|---|---|---|---|
| Kidney | Igfbp2 | 1.65 (0.026†) | 1.76 (0.114) | 2.66 (0.213) |
| Kidney | Igfbp5 | 0.69 (0.024) | 0.78 (0.311) | 1.19 (0.389) |
| Kidney | Igfals | 1.46 (0.001) | 0.29 (< 0.001) | 0.16 (< 0.001) |
| Kidney | Irs1 | 1.42 (0.020) | 1.90 (0.004) | 3.23 (< 0.001) |
| Kidney | Ccnd1 | 1.23 (0.017) | 0.80 (0.154) | 0.69 (0.275) |
| Kidney | Mt1 | 1.23 (0.040) | 1.69 (0.002) | 2.72 (0.024) |
| Liver | Igf2 | 0.64 (0.021) | 1.57 (0.129) | 6.76 (< 0.001) |
| Liver | Igfbp2 | 1.48 (0.026†) | 3.65 (0.051) | 6.24 (< 0.001) |
| Liver | Igfbp7 | 0.57 (0.032) | 1.51 (0.022) | 0.74 (0.095) |
| Heart | Igfbp6 | 1.77 (0.003) | 1.53 (0.061) | 1.17 (0.548) |
| Heart | Igf1 | 1.62 (0.009) | 0.97 (0.932) | 0.37 (0.045) |
| Heart | Igf2 | 1.40 (0.026†) | 0.76 (0.393) | 2.66 (0.009) |
| Lung | Igf1r | 0.66 (0.011) | 0.90 (0.776) | 2.21 (< 0.001) |
| Muscle | Igfbp1 | 1.54 (0.003) | 0.99 (0.954) | 0.75 (0.575) |
| Muscle | Sgk1 | 1.47 (0.028) | 0.88 (0.638) | 0.55 (0.041) |
| Cortex | Sgk1 | 1.57 (0.038) | 0.80 (0.154) | 1.09 (0.583) |
P-value generated from non-parametric Kolmogorov-Smirnov test