Figure 1.

HTT transgene and chromosomal location in HD founder transgenic animals (A) Schematic of the full-length human HTT cDNA ligation fragment used for transgenesis. The full-length cDNA includes exons 1–67 of the HTT gene ligated to a 5′ 1.1 kb fragment of the human HTT promotor region. The 3′-UTR is a genomic fragment of the bovine growth hormone (BGH) gene. (B) HD transgenic founder karyograms and predicted chromosome insertion sites. Location of the transgene is marked by yellow–green fluorescence. The chromosomal insertion site for G₀/1 was observed to be only at 1q43 without the necessity of making a karyogram (data not shown). G₀/3 is a very low-grade mosaic with no signals observed above background in any cells (data not shown). The insertion sites for the transgenes in karyograms of the other four animals are shown above.