Figure 5.

Chk2 overexpression does not affect dMARK activity in fly eyes. A myc-tagged Drosophila dMARK was expressed alone or co-expressed with Drosophila Chk2 using the pan-retinal gmr-GAL4 driver. (A) Western blot with an anti-pT408 dMARK antibody (top) or an anti-myc antibody (bottom). The signals were quantified and the phosphorylation levels are shown as a ratio relative to that of flies expressing dMARK alone. No significant difference was detected (n = 4, P > 0.05, Student's t-test). Representative blots are shown. gmr: flies with the gmr-GAL4 driver only. The asterisk indicates a non-specific band. (B–D) External eyes from the gmr-GAL4 driver control (B), transgenic flies overexpressing dMARK alone (C) or flies co-expressing dMARK and Drosophila Chk2 (D). Chk2 overexpression did not enhance dMARK-induced eye degeneration.