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. 2010 Apr 1;120(5):1749–1761. doi: 10.1172/JCI40372

Figure 8. Ferroportin protein levels are increased by GLRX5 depletion only in erythroid precursor cells.

Figure 8

(A) Western blots of FPN1 in GLRX5 siRNA–treated K562 and HeLa S3 cells, in which GLRX5 was knocked down by 4 siRNA oligos (1 to 4). WT, mock, and neg are negative controls. FPN1, ferroportin. α-Tubulin is loading control. (B) qRT-PCR analysis of GLRX5, FPN1a, FPN1b, and total FPN1 transcripts in GLRX5 siRNA–treated K562 cells. Results were verified by the transcript level of actin, and represent the average of 3 repeats. P = 0.0063, 0.011, 0.015, and 0.007 for GLRX5 in oligo 1–4; P = 0.0076, 0.04, 0.025, and 0.017 for FPN1a in oligo 1–4; P = 0.013, 0.034, 0.12, and 0.004 for FPN1b in oligo 1–4; and P = 0.138, 0.0087, 0.021, and 0.0053 for FPN1 total in oligo 1–4. *P < 0.05; **P < 0.01.