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. 2010 Apr 19;120(5):1578–1590. doi: 10.1172/JCI41502

Figure 6. Reactivity of clone 21.30 to stimulation by mutant HEL peptides.

Figure 6

HEL11–27 mutations at positions P5, P8, and P9 as well as a truncated HEL peptide (HEL11–21) were tested at various concentrations in a classic T cell presentation assay using irradiated splenocytes as APCs and clone 21.30 as responder cells. IL-2 release was measured using an IL-2 reporter cell line. Each assay was repeated at least twice. A representative experiment is shown. Values are mean of triplicates ± SD.