Figure 7. Stimulation of clone 21.30 with I-A^g7HEL_11–27 or I-A^g7HEL_11–27 P9 mutants or truncated I-A^g7HEL_11–22.

MHC peptide complexes were coated in 96-well plates at increasing concentrations and used to stimulate clone 21.30 overnight. IL-2 production was measured using an IL-2–dependent reporter line. Values are the mean of triplicates ± SD.