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. 2010 Apr 1;120(5):1708–1721. doi: 10.1172/JCI40794

Figure 1. Generation of animals with a conditional Ngn3 allele.

Figure 1

(A) Schema depicting the Ngn3 locus and the targeting construct. (B) Targeted Ngn3 allele before and after the excision of the FRT flanked “PGK-Neo” selection cassette by the FLP recombinase. Stars in B indicate the position of the 5′- and 3′-external probes used for Southern blot analysis (see Supplemental Figure 1). (A and B) The black boxes indicate the Ngn3 coding sequence. The PGK-Neomycin selection cassette, the loxP, and FRT sites are indicated as well. X, XbaI; Sp, SpeI; E, EcoRI; Pm, PmeI; As, AscI.