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. 2010 Apr 19;120(5):1506–1514. doi: 10.1172/JCI40096

Figure 3. Reduction of plasma insulin inhibits cardiac hypoxia due to pressure overload.

Figure 3

(A) Animals were prepared as described for Figure 2A. Immuno­histo­chemistry using antibodies against platelet and endothelial cell adhesion molecule (dark brown) and dystrophin (light brown) was performed at 2 weeks after operation. Scale bars: 20 μm. (B and C) CSA of cardiomyocytes (B) and relative vascular density (C) were estimated as described in Methods. n = 4–5. (D) Cardiac ischemia (brown) in mice prepared as described for Figure 2A was estimated with a Hypoxyprobe-1. Scale bars: 1 mm. (E) Number of TUNEL-positive cells per 1 × 104 cardiomyocytes. n = 4–6. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01.