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. 2010 Apr 19;120(5):1506–1514. doi: 10.1172/JCI40096

Figure 4. Cardiomyocyte-specific reduction of Insr expression attenuates systolic dysfunction due to pressure overload.

Figure 4

(A) Western blot analysis of Insr expression in the hearts of CIRKO mice (Insrflox/+Cre+) and their littermate controls (control). Graphs indicate relative expression levels of Insr. n = 3. (B) CIRKO mice (Insrflox/+Cre+) or littermate controls were subjected to TAC or sham operation, and components of the insulin signaling pathway in the heart were examined by Western blot analysis at 2 weeks after operation. Graphs indicate relative expression levels of these signaling molecules. n = 3. (C) The heart weight/body weight ratio of animals prepared as described in A was measured at 2 weeks after operation. n = 7–9. (D) Cardiac hypertrophy and systolic function of animals prepared as described in A were assessed by echocardiography at 1 week (IVST) or 2 weeks (FS and LVDs) after operation. Photographs show representative results of echocardiography (M-mode). n = 8–13. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01.