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. 2010 Apr 19;120(5):1506–1514. doi: 10.1172/JCI40096

Figure 6. Reduced activation of Akt attenuates systolic dysfunction due to pressure overload.

Figure 6

(A) Akt1-deficient (Akt1+/–) mice and WT littermates were subjected to TAC or sham operation. Cardiac hypertrophy and systolic function were assessed by echocardiography at 2 weeks after operation. n = 4–6. (B) Immunohistochemistry using antibodies against platelet and endothelial cell adhesion molecules (dark brown) and dystrophin (light brown) was performed at 2 weeks after operation. Scale bars: 20 μm. (C) CSA of cardiomyocytes and relative vascular density were estimated as described in Methods. n = 3. (D) pAkt and Akt levels in the heart at 2 weeks after operation were examined by Western blot analysis. Graphs indicate relative expression levels of pAkt and Akt. n = 3. Data are shown as mean ± SEM. *P < 0.05; **P < 0.01.