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. 2010 Apr 26;120(5):1415–1428. doi: 10.1172/JCI40494

Figure 4. Endogenous and recombinant mGX sPLA2 both regulate spontaneous AR during capacitation.

Figure 4

(A) Spontaneous AR of mGX–/– sperm does not progress beyond 55 minutes of in vitro capacitation, in contrast to WT sperm. Addition of mGX during the last 10 minutes of 90-minute capacitation rescues normal spontaneous AR in mGX–/– sperm. Spontaneous AR was quantified during capacitation at 10, 55, and 90 minutes in mGX+/+ littermate sperm (n = 11) and mGX–/– sperm (n = 6). (B) Recombinant mGX sPLA2 is a potent inducer of AR. Dose-response curve for AR of noncapacitated WT sperm triggered by mGX (n = 3–8). (C) Treatment with mGX sPLA2 (200 nM) for 10 minutes triggers AR of both noncapacitated WT sperm (23.7% ± 1.4% to 68.1% ± 2.1% for control and mGX-treated sperm, respectively; n = 23) and WT sperm capacitated for 90 minutes (46.5% ± 6.3% to 81.8% ± 5.4% for control and mGX-treated sperm, respectively; n = 3).