Figure 8. Exogenous mGX sPLA2 reinforces the effect of endogenous mGX on spontaneous AR and improves fertility outcome.
(A) Sperm used for IVF and briefly pretreated with exogenous mGX present a higher rate of spontaneous AR. WT sperm were incubated during the last 10 minutes of capacitation with mGX alone (200 nM), LY329722 alone (1 μM), or mGX preincubated with LY329722. Only mGX treatment triggered significant AR over the untreated condition (n = 6). (B) Recombinant mGX controls the yield of viable embryos, without changing the rate of fertilization. IVF was performed either with OF1 control sperm or with the same sperm briefly treated with 200 nM mGX, and IVF outcome was scored (n = 13). Recombinant mGX increases 2-cell embryos and decreases aborted embryos. (C) Two-cell embryos obtained with mGX-treated sperm developed normally to the blastocyst stage. (D) LY329722 blocks the mGX-dependent increase in fertility. IVF was performed with either control sperm or the same sperm treated briefly with 200 nM mGX or with the same sperm treated with 200 nM mGX but preincubated with 1 μM LY329722 (n = 6). (E) The number of sperm incubated with oocytes does not impact fertility outcome. IVF was performed at 2 different concentrations: 1.5 × 105 and 3 × 105 sperm (sp) (n = 4).