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. 2010 Apr 26;120(5):1617–1626. doi: 10.1172/JCI41678

Figure 3. Oxidized linoleic acid metabolites are TRPV1 agonists.

Figure 3

(A and B) Synthetic 9-HODE (100 μM) activates TG neurons from WT mice but not from TRPV1 KO mice as measured using calcium imaging. (C) Graph summarizing comparison of 9-HODE activation of TG neurons from WT versus TRPV1 KO mice (n = 50 for WT and 66 for TRPV1 KO; P = 0.0002). (D) Whole-cell recording demonstrates activation of a rat TG neuron by synthetic 9-HODE (100 μM) and capsaicin. (E) Concentration-response effects of applying synthetic 9-HODE (15 minutes) on iCGRP release from cultured rat TG neurons as measured by radioimmunoassay (n = 8–16 wells/group; P = 0.0001). BL, baseline. (F) Concentration-response curve for synthetic 13-HODE. Responses (pA/pF) were recorded from TRPV1-expressing CHO cells. (G) Effect of applying synthetic 9-oxoODE (100 μM) on TG neurons from WT mice (n = 71 for WT and 83 for TRPV1 KO; P = 0.0007). *P < 0.05; **P < 0.01; ***P < 0.001. (H and I) Comparison of the effects of applying linoleic acid metabolites (all 100 μM; n = 47–75) to TG neurons cultured from WT mice (H) or to CHO cells transfected with TRPV1 (I) as measured by calcium imaging.